Primers for low‐copy nuclear genes in Metrosideros and cross‐amplification in Myrtaceae

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Primers for low-copy nuclear genes in Metrosideros and cross-amplification in Myrtaceae1

UNLABELLED PREMISE OF THE STUDY Primers were developed to amplify low-copy nuclear genes in Hawaiian Metrosideros (Myrtaceae). • METHODS AND RESULTS Data from a pooled 454 Titanium run of the partial transcriptomes of four Metrosideros taxa were used to identify the loci of interest. Ten exon-primed intron-crossing (EPIC) markers were amplified and sequenced directly with success in Metros...

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ژورنال

عنوان ژورنال: Applications in Plant Sciences

سال: 2014

ISSN: 2168-0450,2168-0450

DOI: 10.3732/apps.1400049